Detailed information of TA system TA104473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 481622..482316
• Replicon: chromosome
• Accession: NZ_CP028950
• Organism: Enterobacter cloacae complex sp. FDA-CDC-AR_0164

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: AM410_RS02385
• Protein ID: WP_175556434.1
• Coordinates: 481622..482020 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: A0A285AZA0
• Locus tag: AM410_RS02390
• Protein ID: WP_073395581.1
• Coordinates: 482023..482316 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM410_RS02365	477609..478373	+	765	WP_014168780.1	galactose-binding lectin	-
AM410_RS02370	478437..479843	+	1407	WP_073395587.1	carbohydrate binding domain-containing protein	-
AM410_RS02375	480028..480720	-	693	WP_073395586.1	hydrolase	-
AM410_RS02380	480829..481470	+	642	WP_073395584.1	TetR/AcrR family transcriptional regulator	-
AM410_RS02385	481622..482020	-	399	WP_175556434.1	type II toxin-antitoxin system YafO family toxin	Toxin
AM410_RS02390	482023..482316	-	294	WP_073395581.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AM410_RS02395	482856..483755	+	900	WP_073395579.1	DUF4365 domain-containing protein	-
AM410_RS02400	483839..484738	+	900	Protein_458	hypothetical protein	-
AM410_RS02405	484896..485633	+	738	Protein_459	DUF3644 domain-containing protein	-
AM410_RS02410	485642..486888	-	1247	Protein_460	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15314.63 Da
• Isoelectric Point: 7.1157

>T104473 WP_175556434.1 NZ_CP028950:c482020-481622 [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
IRIFKSALIRQQLSQQELDDLAADFLSYKKDCVLPDTFGRDAPYDDDRTYPLVKEEQVAHIHLADADAPFPKFLRQFKRT
SDQAHLVYCQGAMDPDAYLLIIILKPEAHKMARNNNHMHKIGMMAEAFRMKH

Nucleotide

• Length: 399 bp

>T104473 NZ_CP028950:c482020-481622 [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
ATCAGGATTTTCAAGTCAGCACTGATCCGTCAACAATTAAGCCAACAAGAGCTTGATGATTTGGCGGCGGATTTTCTGTC
CTATAAAAAGGATTGCGTGTTGCCAGATACCTTTGGGCGTGATGCGCCTTATGATGATGACCGCACCTATCCCTTGGTAA
AAGAAGAGCAAGTGGCCCACATTCATCTTGCCGATGCGGATGCCCCTTTCCCGAAGTTTTTACGTCAGTTCAAACGAACC
AGCGATCAGGCGCATTTGGTATACTGCCAAGGTGCGATGGACCCTGATGCCTATCTGTTGATTATCATTCTGAAACCCGA
AGCGCATAAAATGGCGCGTAACAATAACCATATGCATAAGATCGGGATGATGGCCGAAGCGTTCAGGATGAAGCATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10979.44 Da
• Isoelectric Point: 4.9167

>AT104473 WP_073395581.1 NZ_CP028950:c482316-482023 [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEEKQGRVHTGARFRPTAERLSDIADSG
QKLLQNASDKDLTEFTE

Nucleotide

• Length: 294 bp

>AT104473 NZ_CP028950:c482316-482023 [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
ATGGAACGTATCCTCGCAGAAAAATCTATCAACATCACCGAGTTACGAAAGAACCCGGCGAAATATTTTATTGATGAACC
GGTTGCCGTTCTCTCAAACAACCGTCCCGCTGGCTACATGGTTAGTGCTAAGGTGTTTGAAGAGCTGATCGATCTGTTGG
AAGAGAAGCAGGGCAGGGTTCATACCGGTGCGCGATTCAGGCCAACGGCTGAACGTTTAAGTGATATTGCGGATAGTGGA
CAGAAACTGCTGCAAAACGCCTCTGACAAGGATCTGACCGAGTTCACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A285AZA0