Detailed information of TA system TA104433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 934211..934864
• Replicon: chromosome
• Accession: NZ_CP028948
• Organism: Serratia marcescens strain AR_0123

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM370_RS04620
• Protein ID: WP_033645671.1
• Coordinates: 934514..934864 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: AM370_RS04615
• Protein ID: WP_019453270.1
• Coordinates: 934211..934510 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM370_RS04590	929632..930555	-	924	WP_033648563.1	3-hydroxyacyl-CoA dehydrogenase family protein	-
AM370_RS04595	930574..931758	-	1185	WP_033648565.1	acetyl-CoA C-acetyltransferase	-
AM370_RS04600	931772..932425	-	654	WP_033648566.1	CoA transferase subunit B	-
AM370_RS04605	932437..933135	-	699	WP_033648567.1	CoA transferase subunit A	-
AM370_RS04610	933304..934221	+	918	WP_033648568.1	LysR family transcriptional regulator	-
AM370_RS04615	934211..934510	-	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
AM370_RS04620	934514..934864	-	351	WP_033645671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM370_RS04625	935136..936140	+	1005	WP_043148198.1	hypothetical protein	-
AM370_RS04630	936140..936535	+	396	WP_033648570.1	hypothetical protein	-
AM370_RS04635	936526..936843	-	318	WP_041037744.1	hypothetical protein	-
AM370_RS04640	937089..938459	+	1371	WP_033648572.1	glutamine synthetase family protein	-
AM370_RS04645	938489..938899	+	411	WP_033648574.1	GNAT family N-acetyltransferase	-
AM370_RS04650	938913..939602	+	690	WP_033648575.1	trehalose-6-phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13546.81 Da
• Isoelectric Point: 8.2699

>T104433 WP_033645671.1 NZ_CP028948:c934864-934514 [Serratia marcescens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T104433 NZ_CP028948:c934864-934514 [Serratia marcescens]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAGCAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCCGGGCCTTCTCTCCGGCGGCCTTTTGTCGACGTGCTGAAAGGTTCGTTGCATCCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCCTTTTTCGCCTTCGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATTGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT104433 WP_019453270.1 NZ_CP028948:c934510-934211 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT104433 NZ_CP028948:c934510-934211 [Serratia marcescens]
ATGAGCACCTTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGCTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCGGTCGCGGCCATCGAAGGGCGCGGCAACGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3