Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4858394..4858896 Replicon chromosome
Accession NZ_CP028917
Organism Pseudomonas aeruginosa strain JB2

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1C7CBF7
Locus tag C5B40_RS23120 Protein ID WP_023081358.1
Coordinates 4858394..4858648 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A1C7C600
Locus tag C5B40_RS23125 Protein ID WP_004350479.1
Coordinates 4858645..4858896 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B40_RS23100 4854829..4855692 + 864 WP_004350470.1 integrase domain-containing protein -
C5B40_RS23105 4855880..4856230 + 351 WP_023081356.1 hypothetical protein -
C5B40_RS23110 4856245..4857765 + 1521 WP_004350477.1 conjugal transfer protein TraG N-terminal domain-containing protein -
C5B40_RS23115 4857773..4858168 - 396 WP_023081357.1 DUF3742 family protein -
C5B40_RS23120 4858394..4858648 - 255 WP_023081358.1 Txe/YoeB family addiction module toxin Toxin
C5B40_RS23125 4858645..4858896 - 252 WP_004350479.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C5B40_RS23130 4858981..4860351 - 1371 WP_023081359.1 tyrosine-type recombinase/integrase -
C5B40_RS23140 4861539..4862294 + 756 WP_003460276.1 hypothetical protein -
C5B40_RS23145 4862414..4862626 + 213 WP_003460278.1 AlpA family transcriptional regulator -
C5B40_RS23150 4862670..4863545 + 876 WP_011489277.1 ParA family protein -
C5B40_RS23155 4863529..4863786 + 258 WP_003460282.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4849671..4869403 19732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10061.50 Da        Isoelectric Point: 8.4789

>T104370 WP_023081358.1 NZ_CP028917:c4858648-4858394 [Pseudomonas aeruginosa]
VKLIWSEQAWDDYLYWQDTDKRMVKKVNELIKDACRTPFEGKGKPEPLRHNLAGFWSRRITAEHRFVYSVTDDALLIAAC
RYHY

Download         Length: 255 bp

>T104370 NZ_CP028917:c4858648-4858394 [Pseudomonas aeruginosa]
GTGAAGCTGATCTGGTCGGAACAAGCCTGGGACGATTATCTGTACTGGCAGGACACGGATAAGCGTATGGTGAAAAAGGT
CAACGAGTTGATCAAGGATGCCTGCCGTACCCCGTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGACGGATCACGGCCGAGCATCGGTTTGTGTATTCGGTGACGGATGACGCGCTGCTGATTGCAGCCTGC
CGTTACCACTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9363.65 Da        Isoelectric Point: 4.5843

>AT104370 WP_004350479.1 NZ_CP028917:c4858896-4858645 [Pseudomonas aeruginosa]
MRTISYTEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEFNALEETAYLLRSPANARRLMDSIDSLKQGQGVERD
LAE

Download         Length: 252 bp

>AT104370 NZ_CP028917:c4858896-4858645 [Pseudomonas aeruginosa]
ATGCGCACGATCAGTTACACCGAAGCCCGGCAGAACCTGTCGGCCACCATGATGAAGACCGTGGAGGATCGCGCCCCGAT
CCTGATTACCCGGCAGAACGGCGAAGCCTGCGTGTTGATGTCCCTGGAAGAGTTCAACGCGCTGGAGGAAACGGCTTATC
TGCTGCGCTCCCCGGCCAATGCCCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCAAGGCCAGGGCGTCGAGCGGGAT
TTGGCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C7CBF7

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7C600

References