104327

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 433364..433979 Replicon chromosome
Accession NZ_CP028893
Organism Vibrio cholerae strain Sa5Y

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag Sa5Y_RS16670 Protein ID WP_162298939.1
Coordinates 433364..433651 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag Sa5Y_RS16675 Protein ID WP_001232701.1
Coordinates 433662..433979 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Sa5Y_RS16595 428743..428925 + 183 Protein_462 DUF645 family protein -
Sa5Y_RS16600 429128..429625 + 498 WP_033933253.1 GNAT family N-acetyltransferase -
Sa5Y_RS16605 429745..430200 + 456 WP_001245329.1 GNAT family N-acetyltransferase -
Sa5Y_RS16615 430337..430669 + 333 WP_039470247.1 hypothetical protein -
Sa5Y_RS16620 430642..430776 + 135 Protein_466 DUF3265 domain-containing protein -
Sa5Y_RS16625 430808..431236 + 429 WP_069648560.1 ester cyclase -
Sa5Y_RS16635 431466..431891 + 426 WP_094393057.1 hypothetical protein -
Sa5Y_RS16645 432022..432339 - 318 WP_000077449.1 CcdB family protein -
Sa5Y_RS16650 432339..432584 - 246 WP_001260797.1 type II toxin-antitoxin system CcdA family antitoxin -
Sa5Y_RS16660 432783..433136 + 354 WP_095488391.1 hypothetical protein -
Sa5Y_RS16670 433364..433651 + 288 WP_162298939.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Sa5Y_RS16675 433662..433979 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
Sa5Y_RS16685 434479..434649 + 171 Protein_474 DUF645 family protein -
Sa5Y_RS16690 434867..435997 + 1131 WP_094393023.1 DUF4238 domain-containing protein -
Sa5Y_RS16695 436201..436590 + 390 WP_000491261.1 VOC family protein -
Sa5Y_RS20240 436675..436794 + 120 WP_162843095.1 acetyltransferase -
Sa5Y_RS16705 436778..437014 - 237 WP_000772130.1 RNA-binding protein -
Sa5Y_RS16715 438030..438287 + 258 WP_055031549.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
Sa5Y_RS16720 438275..438577 + 303 WP_095486992.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 306104..472928 166824
- inside Integron - - 307268..474111 166843


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11362.94 Da        Isoelectric Point: 6.7187

>T104327 WP_162298939.1 NZ_CP028893:433364-433651 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNNYLDPHKY

Download         Length: 288 bp

>T104327 NZ_CP028893:433364-433651 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATAATTACTTAGACCCACATAAGTATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT104327 WP_001232701.1 NZ_CP028893:433662-433979 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT104327 NZ_CP028893:433662-433979 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGGGTACACAGAAATACGGTGAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACCCCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References