Detailed information of TA system TA104315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 166396..167123
• Replicon: plasmid pVIR_3214
• Accession: NZ_CP028852
• Organism: Klebsiella pneumoniae strain 3214

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: DBX64_RS28390
• Protein ID: WP_011251285.1
• Coordinates: 166396..166707 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBX64_RS28395
• Protein ID: WP_011251286.1
• Coordinates: 166704..167123 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBX64_RS28350	161457..161972	-	516	WP_041937820.1	hypothetical protein	-
DBX64_RS28355	162080..162655	-	576	WP_074424927.1	hypothetical protein	-
DBX64_RS28360	162772..163728	-	957	WP_011251280.1	DsbA family protein	-
DBX64_RS28365	163788..164129	-	342	WP_011251281.1	hypothetical protein	-
DBX64_RS28370	164143..164454	-	312	WP_011251282.1	hypothetical protein	-
DBX64_RS28375	164471..164905	-	435	Protein_180	thioredoxin fold domain-containing protein	-
DBX64_RS28380	164922..165619	-	698	Protein_181	IS1 family transposase	-
DBX64_RS28385	165733..166191	+	459	Protein_182	DDE-type integrase/transposase/recombinase	-
DBX64_RS28390	166396..166707	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
DBX64_RS28395	166704..167123	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
DBX64_RS28400	167270..168238	+	969	WP_074428168.1	IS5 family transposase	-
DBX64_RS28405	168340..168675	-	336	Protein_186	conjugal transfer protein TraI	-
DBX64_RS28410	168689..169477	-	789	WP_040217257.1	hypothetical protein	-
DBX64_RS28415	169498..169941	-	444	WP_048333569.1	hypothetical protein	-
DBX64_RS28420	170537..171292	+	756	Protein_189	hypothetical protein	-
DBX64_RS28425	171485..171955	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..231431	231431
	-	inside	IScluster/Tn	-	-	164922..177244	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T104315 WP_011251285.1 NZ_CP028852:166396-166707 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T104315 NZ_CP028852:166396-166707 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT104315 WP_011251286.1 NZ_CP028852:166704-167123 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT104315 NZ_CP028852:166704-167123 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1