Detailed information of TA system TA104295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5431444..5432039
• Replicon: chromosome
• Accession: NZ_CP028849
• Organism: Pseudomonas aeruginosa strain IMP68

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: DBZ62_RS25940
• Protein ID: WP_003113526.1
• Coordinates: 5431761..5432039 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBZ62_RS25935
• Protein ID: WP_003113527.1
• Coordinates: 5431444..5431749 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBZ62_RS25900	5426583..5427431	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
DBZ62_RS25910	5427598..5428539	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
DBZ62_RS25915	5428656..5429270	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
DBZ62_RS25920	5429312..5429896	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
DBZ62_RS25925	5429937..5431037	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
DBZ62_RS25935	5431444..5431749	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
DBZ62_RS25940	5431761..5432039	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBZ62_RS25950	5432368..5434596	+	2229	WP_003099265.1	TonB-dependent receptor	-
DBZ62_RS25955	5434666..5435313	-	648	WP_003095021.1	carbonate dehydratase	-
DBZ62_RS25960	5435375..5436613	-	1239	WP_033970863.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T104295 WP_003113526.1 NZ_CP028849:c5432039-5431761 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T104295 NZ_CP028849:c5432039-5431761 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT104295 WP_003113527.1 NZ_CP028849:c5431749-5431444 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT104295 NZ_CP028849:c5431749-5431444 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3