Detailed information of TA system TA104239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 15843..16500
• Replicon: plasmid pEI-MS-17-156-1
• Accession: NZ_CP028814
• Organism: Edwardsiella ictaluri strain MS-17-156

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: DBV23_RS18445
• Protein ID: WP_000270043.1
• Coordinates: 15843..16193 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBV23_RS18450
• Protein ID: WP_000124640.1
• Coordinates: 16198..16500 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBV23_RS19460	11531..11830	+	300	WP_159075504.1	hypothetical protein	-
DBV23_RS18405	12317..12814	-	498	WP_000062185.1	hypothetical protein	-
DBV23_RS18410	12817..13305	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
DBV23_RS18415	13402..13737	+	336	WP_000683476.1	hypothetical protein	-
DBV23_RS18420	13752..14222	-	471	WP_001281821.1	hypothetical protein	-
DBV23_RS18425	14215..14586	-	372	WP_000516916.1	hypothetical protein	-
DBV23_RS18430	14597..14791	-	195	WP_000343597.1	hypothetical protein	-
DBV23_RS18440	15132..15680	-	549	WP_001061574.1	transcriptional regulator	-
DBV23_RS18445	15843..16193	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBV23_RS18450	16198..16500	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
DBV23_RS18455	16527..16820	-	294	WP_001239997.1	chromosome segregation protein ParM	-
DBV23_RS18460	16908..17180	-	273	WP_001043047.1	HU family DNA-binding protein	-
DBV23_RS18465	17238..17765	-	528	WP_001236377.1	thermonuclease family protein	-
DBV23_RS18475	17996..18853	-	858	WP_001167032.1	hypothetical protein	-
DBV23_RS18480	18840..19070	-	231	WP_000972663.1	hypothetical protein	-
DBV23_RS18485	19070..19588	-	519	WP_000210756.1	nitrite reductase	-
DBV23_RS18490	19585..20031	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
DBV23_RS18495	20031..20390	-	360	WP_000422768.1	hypothetical protein	-
DBV23_RS18500	20447..20875	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(D) / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..135268	135268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T104239 WP_000270043.1 NZ_CP028814:15843-16193 [Edwardsiella ictaluri]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T104239 NZ_CP028814:15843-16193 [Edwardsiella ictaluri]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT104239 WP_000124640.1 NZ_CP028814:16198-16500 [Edwardsiella ictaluri]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT104239 NZ_CP028814:16198-16500 [Edwardsiella ictaluri]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9