Detailed information of TA system TA104237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3564458..3565085
• Replicon: chromosome
• Accession: NZ_CP028813
• Organism: Edwardsiella ictaluri strain MS-17-156

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A411H7J1
• Locus tag: DBV23_RS17010
• Protein ID: WP_015871332.1
• Coordinates: 3564756..3565085 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: C5BBM8
• Locus tag: DBV23_RS17005
• Protein ID: WP_015871333.1
• Coordinates: 3564458..3564766 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBV23_RS16970	3560378..3560725	+	348	WP_015871339.1	hypothetical protein	-
DBV23_RS16975	3560835..3561479	+	645	WP_015871337.1	DUF1367 family protein	-
DBV23_RS16980	3561482..3561781	+	300	WP_015871336.1	DUF1364 domain-containing protein	-
DBV23_RS16985	3561926..3562966	+	1041	WP_015871335.1	hypothetical protein	-
DBV23_RS16990	3562967..3563476	+	510	WP_071525693.1	hypothetical protein	-
DBV23_RS17000	3563843..3564355	-	513	WP_015871334.1	DUF2442 domain-containing protein	-
DBV23_RS17005	3564458..3564766	-	309	WP_015871333.1	helix-turn-helix transcriptional regulator	Antitoxin
DBV23_RS17010	3564756..3565085	-	330	WP_015871332.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBV23_RS17015	3565534..3566097	+	564	Protein_3238	Rha family transcriptional regulator	-
DBV23_RS17020	3566438..3566953	+	516	WP_034165088.1	hypothetical protein	-
DBV23_RS17025	3567042..3567860	+	819	WP_015871329.1	hypothetical protein	-
DBV23_RS17030	3567966..3568210	+	245	Protein_3241	transposase	-
DBV23_RS17035	3568342..3568572	+	231	WP_015871327.1	ParD-like family protein	-
DBV23_RS17040	3568547..3568876	+	330	WP_015871326.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DBV23_RS17045	3568960..3569307	+	348	WP_035608895.1	phage holin, lambda family	-
DBV23_RS17050	3569310..3569861	+	552	WP_015871324.1	glycoside hydrolase family 108 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3520125..3610184	90059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12771.78 Da
• Isoelectric Point: 9.6247

>T104237 WP_015871332.1 NZ_CP028813:c3565085-3564756 [Edwardsiella ictaluri]
MNYTIEYYSEEVRLEVDQLPMGMRVRYQHLVERMEIYGSNLGEPHTSPFGDGLFELRIKGSDGIARVFYCTLTGKRIVML
HSFIKKTQKTPSAERKKAETRMKEVKHGW

Nucleotide

• Length: 330 bp

>T104237 NZ_CP028813:c3565085-3564756 [Edwardsiella ictaluri]
ATGAACTACACTATTGAGTACTACAGCGAAGAGGTTCGGCTTGAGGTCGATCAGTTGCCAATGGGTATGCGGGTTCGATA
CCAGCATCTCGTTGAACGCATGGAAATCTACGGTAGTAATCTCGGAGAACCACACACAAGCCCTTTTGGCGATGGGCTTT
TTGAGCTTCGAATCAAAGGCAGTGATGGCATCGCCCGCGTGTTTTACTGCACTCTTACCGGAAAACGTATCGTCATGCTG
CACAGCTTCATCAAGAAAACCCAGAAGACGCCAAGCGCTGAACGTAAGAAGGCTGAAACCAGAATGAAGGAGGTAAAACA
TGGCTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11207.86 Da
• Isoelectric Point: 8.5371

>AT104237 WP_015871333.1 NZ_CP028813:c3564766-3564458 [Edwardsiella ictaluri]
MAGKRTPPTMTHDEMAAKWMEDPAFKAEYDAIEEEYALLDEMLAARKNAGLTQAQVAERMGTKATAITRMESNLASGQSG
PSFATLKKFAHATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT104237 NZ_CP028813:c3564766-3564458 [Edwardsiella ictaluri]
ATGGCTGGTAAACGCACCCCCCCCACGATGACGCATGATGAAATGGCTGCAAAATGGATGGAAGACCCGGCATTTAAAGC
AGAATACGATGCCATCGAAGAGGAATACGCCCTACTCGATGAAATGCTCGCAGCCCGCAAAAACGCGGGGCTGACCCAGG
CGCAGGTCGCCGAACGTATGGGAACCAAGGCTACAGCGATCACTCGCATGGAAAGTAATTTAGCCTCCGGCCAAAGCGGG
CCATCATTCGCCACGCTGAAAAAATTTGCACACGCGACCGGCAAGAAACTGCAGATCCGGTTTGTCTGA

Structures

Toxin

• AlphaFold DB: A0A411H7J1

Antitoxin

• AlphaFold DB: C5BBM8