Detailed information of TA system TA104198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 17293..17878
• Replicon: plasmid p1_010059
• Accession: NZ_CP028798
• Organism: Acinetobacter junii strain WCHAJ59

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: CDG57_RS00115
• Protein ID: WP_000897307.1
• Coordinates: 17558..17878 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDG57_RS00110
• Protein ID: WP_000369781.1
• Coordinates: 17293..17565 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG57_RS00080	12333..12974	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
CDG57_RS00090	13587..13817	+	231	WP_005155998.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CDG57_RS00095	13814..14209	+	396	WP_012268403.1	type II toxin-antitoxin system VapC family toxin	-
CDG57_RS00100	14382..15266	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
CDG57_RS00105	15322..16797	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
CDG57_RS00110	17293..17565	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
CDG57_RS00115	17558..17878	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG57_RS00120	18044..18583	-	540	WP_002055813.1	hypothetical protein	-
CDG57_RS00125	18608..19447	-	840	WP_001058024.1	alpha/beta fold hydrolase	-
CDG57_RS00130	19540..20130	+	591	WP_000058345.1	TetR/AcrR family transcriptional regulator	-
CDG57_RS00140	20433..20999	-	567	WP_000678311.1	recombinase family protein	-
CDG57_RS00145	21492..21641	+	150	WP_079878061.1	DUF4113 domain-containing protein	-
CDG57_RS00150	21802..22089	-	288	WP_005130374.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CDG57_RS00155	22079..22327	-	249	WP_005130375.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CDG57_RS00160	22330..22755	-	426	WP_005130376.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / tet(39) / mph(E) / msr(E)	-	1..33869	33869
	-	inside	IScluster/Tn	aac(3)-IId / tet(39) / mph(E) / msr(E)	-	1802..16797	14995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T104198 WP_000897307.1 NZ_CP028798:c17878-17558 [Acinetobacter junii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T104198 NZ_CP028798:c17878-17558 [Acinetobacter junii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT104198 WP_000369781.1 NZ_CP028798:c17565-17293 [Acinetobacter junii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT104198 NZ_CP028798:c17565-17293 [Acinetobacter junii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3