Detailed information of TA system TA104174

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 111371..111624
• Replicon: plasmid pKPC2_040035
• Accession: NZ_CP028796
• Organism: Klebsiella pneumoniae strain WCHKP040035

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: C6M51_RS01220
• Protein ID: WP_001312851.1
• Coordinates: 111475..111624 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	111371..111430 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M51_RS01170	106467..106571	+	105	Protein_167	transcriptional regulator	-
C6M51_RS01175	106594..106791	-	198	Protein_168	hypothetical protein	-
C6M51_RS01180	107149..107853	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C6M51_RS01185	107917..108078	+	162	Protein_170	DNA helicase	-
C6M51_RS01190	108098..108844	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
C6M51_RS01195	108899..109459	+	561	WP_000139328.1	fertility inhibition protein FinO	-
C6M51_RS01200	109591..109791	+	201	WP_015059022.1	hypothetical protein	-
C6M51_RS01205	110177..110776	+	600	WP_032083981.1	hypothetical protein	-
C6M51_RS01210	110940..111170	+	231	WP_001736714.1	hypothetical protein	-
C6M51_RS01215	111193..111420	-	228	Protein_176	hypothetical protein	-
-	111371..111430	-	60	NuclAT_1	-	Antitoxin
-	111371..111430	-	60	NuclAT_1	-	Antitoxin
-	111371..111430	-	60	NuclAT_1	-	Antitoxin
-	111371..111430	-	60	NuclAT_1	-	Antitoxin
C6M51_RS01220	111475..111624	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
C6M51_RS01225	111908..112156	+	249	WP_000083837.1	replication regulatory protein RepA	-
C6M51_RS01230	112157..112366	-	210	WP_064765370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..112467	112467
	-	flank	IS/Tn	-	-	107149..107853	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T104174 WP_001312851.1 NZ_CP028796:111475-111624 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T104174 NZ_CP028796:111475-111624 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT104174 NZ_CP028796:c111430-111371 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin