Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 51251..51978 Replicon plasmid pQnrS1_020030
Accession NZ_CP028792
Organism Klebsiella pneumoniae strain WCHKP020030

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag C6M02_RS02920 Protein ID WP_004118600.1
Coordinates 51251..51562 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag C6M02_RS02925 Protein ID WP_000990392.1
Coordinates 51559..51978 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M02_RS02900 47765..48484 + 720 WP_001102107.1 (Fe-S)-binding protein -
C6M02_RS02905 48495..49922 + 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
C6M02_RS02910 49915..50610 + 696 WP_004118595.1 lactate utilization protein C -
C6M02_RS02915 50660..51097 - 438 WP_003031542.1 gluconate transporter -
C6M02_RS02920 51251..51562 + 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
C6M02_RS02925 51559..51978 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
C6M02_RS02930 52015..53217 - 1203 WP_003031541.1 hypothetical protein -
C6M02_RS02935 53210..53539 - 330 WP_004118613.1 hypothetical protein -
C6M02_RS02940 53536..54057 - 522 WP_000611681.1 hypothetical protein -
C6M02_RS02945 54389..54619 - 231 WP_000124733.1 hypothetical protein -
C6M02_RS02950 54619..54975 - 357 WP_077253206.1 recombination enhancement function domain protein -
C6M02_RS02955 55528..55881 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
C6M02_RS02960 55929..56045 + 117 Protein_59 arsenical resistance operon transcriptional repressor ArsD -
C6M02_RS02965 56104..56808 + 705 WP_001067855.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / dfrA1 / qacE / sul1 / mph(A) / aph(3')-Ia / blaSHV-12 / qnrS1 / blaLAP-2 / aac(3)-IId - 1..125009 125009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T104154 WP_004118600.1 NZ_CP028792:51251-51562 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T104154 NZ_CP028792:51251-51562 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT104154 WP_000990392.1 NZ_CP028792:51559-51978 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT104154 NZ_CP028792:51559-51978 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References