Detailed information of TA system TA104107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4845693..4846295
• Replicon: chromosome
• Accession: NZ_CP028772
• Organism: Escherichia coli strain A2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY53_RS23540
• Protein ID: WP_000897305.1
• Coordinates: 4845984..4846295 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY53_RS23535
• Protein ID: WP_000356397.1
• Coordinates: 4845693..4845983 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY53_RS23510 (4841637)	4841637..4842539	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY53_RS23515 (4842536)	4842536..4843171	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY53_RS23520 (4843168)	4843168..4844097	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY53_RS23525 (4844427)	4844427..4844669	-	243	WP_001087409.1	protein YiiF	-
AOY53_RS23530 (4844889)	4844889..4845107	-	219	WP_001315930.1	CopG family transcriptional regulator	-
AOY53_RS23535 (4845693)	4845693..4845983	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY53_RS23540 (4845984)	4845984..4846295	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY53_RS23545 (4846524)	4846524..4847432	+	909	WP_001162704.1	alpha/beta hydrolase	-
AOY53_RS23550 (4847496)	4847496..4848437	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY53_RS23555 (4848482)	4848482..4848919	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY53_RS23560 (4848916)	4848916..4849788	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY53_RS23565 (4849782)	4849782..4850381	-	600	WP_001295269.1	glucose-1-phosphatase	-
AOY53_RS23570 (4850480)	4850480..4851265	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T104107 WP_000897305.1 NZ_CP028772:c4846295-4845984 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T104107 NZ_CP028772:c4846295-4845984 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT104107 WP_000356397.1 NZ_CP028772:c4845983-4845693 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT104107 NZ_CP028772:c4845983-4845693 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7