Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 643662..644461 Replicon chromosome
Accession NZ_CP028772
Organism Escherichia coli strain A2

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag AOY53_RS03135 Protein ID WP_000347267.1
Coordinates 643662..644126 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag AOY53_RS03140 Protein ID WP_001307405.1
Coordinates 644126..644461 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY53_RS03105 (638663) 638663..639097 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
AOY53_RS03110 (639115) 639115..639993 - 879 WP_001380079.1 PTS N-acetylgalactosamine transporter subunit IID -
AOY53_RS03115 (639983) 639983..640762 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
AOY53_RS03120 (640773) 640773..641246 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
AOY53_RS03125 (641269) 641269..642549 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
AOY53_RS03130 (642798) 642798..643607 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AOY53_RS03135 (643662) 643662..644126 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AOY53_RS03140 (644126) 644126..644461 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AOY53_RS03145 (644610) 644610..646181 - 1572 WP_001273741.1 galactarate dehydratase -
AOY53_RS03150 (646556) 646556..647890 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
AOY53_RS03155 (647906) 647906..648676 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 643662..655117 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T104084 WP_000347267.1 NZ_CP028772:c644126-643662 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T104084 NZ_CP028772:c644126-643662 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT104084 WP_001307405.1 NZ_CP028772:c644461-644126 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT104084 NZ_CP028772:c644461-644126 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References