Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3624213..3624940 Replicon chromosome
Accession NZ_CP028771
Organism Escherichia coli strain A3

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag AOY54_RS17560 Protein ID WP_016233494.1
Coordinates 3624629..3624940 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AOY54_RS17555 Protein ID WP_000126294.1
Coordinates 3624213..3624632 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY54_RS17540 (3620376) 3620376..3622628 - 2253 WP_001350159.1 carbamoyltransferase HypF -
AOY54_RS17545 (3622781) 3622781..3623308 - 528 WP_001078777.1 electron transport protein HydN -
AOY54_RS17550 (3623693) 3623693..3624121 + 429 WP_000536064.1 hypothetical protein -
AOY54_RS17555 (3624213) 3624213..3624632 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
AOY54_RS17560 (3624629) 3624629..3624940 - 312 WP_016233494.1 type II toxin-antitoxin system HigB family toxin Toxin
AOY54_RS17565 (3625106) 3625106..3625576 - 471 WP_000132959.1 hydrogenase maturation peptidase HycI -
AOY54_RS17570 (3625569) 3625569..3625979 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
AOY54_RS17575 (3625976) 3625976..3626743 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
AOY54_RS17580 (3626743) 3626743..3627285 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
AOY54_RS17585 (3627295) 3627295..3629004 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12453.25 Da        Isoelectric Point: 9.7075

>T104080 WP_016233494.1 NZ_CP028771:c3624940-3624629 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNKLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T104080 NZ_CP028771:c3624940-3624629 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAAGCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT104080 WP_000126294.1 NZ_CP028771:c3624632-3624213 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT104080 NZ_CP028771:c3624632-3624213 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGATACTAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References