Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2305790..2306343 Replicon chromosome
Accession NZ_CP028771
Organism Escherichia coli strain A3

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag AOY54_RS11155 Protein ID WP_000605680.1
Coordinates 2306074..2306343 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY54_RS11150 Protein ID WP_000781370.1
Coordinates 2305790..2306074 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY54_RS11135 (2300971) 2300971..2304018 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
AOY54_RS11140 (2304031) 2304031..2304915 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AOY54_RS11145 (2304908) 2304908..2305561 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY54_RS11150 (2305790) 2305790..2306074 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY54_RS11155 (2306074) 2306074..2306343 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY54_RS11160 (2306529) 2306529..2307539 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AOY54_RS11165 (2307673) 2307673..2309370 - 1698 WP_000433460.1 malate dehydrogenase -
AOY54_RS11170 (2309527) 2309527..2309664 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY54_RS11175 (2309766) 2309766..2309981 - 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY54_RS11180 (2310326) 2310326..2310757 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T104069 WP_000605680.1 NZ_CP028771:c2306343-2306074 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T104069 NZ_CP028771:c2306343-2306074 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT104069 WP_000781370.1 NZ_CP028771:c2306074-2305790 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT104069 NZ_CP028771:c2306074-2305790 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References