Detailed information of TA system TA104058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 170387..170989
• Replicon: chromosome
• Accession: NZ_CP028771
• Organism: Escherichia coli strain A3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AOY54_RS00810
• Protein ID: WP_000897302.1
• Coordinates: 170387..170698 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY54_RS00815
• Protein ID: WP_000356397.1
• Coordinates: 170699..170989 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY54_RS00780 (165417)	165417..166202	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY54_RS00785 (166301)	166301..166900	+	600	WP_001314324.1	glucose-1-phosphatase	-
AOY54_RS00790 (166894)	166894..167766	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY54_RS00795 (167763)	167763..168200	+	438	WP_000560978.1	D-aminoacyl-tRNA deacylase	-
AOY54_RS00800 (168245)	168245..169186	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY54_RS00805 (169250)	169250..170158	-	909	WP_001331553.1	alpha/beta hydrolase	-
AOY54_RS00810 (170387)	170387..170698	+	312	WP_000897302.1	hypothetical protein	Toxin
AOY54_RS00815 (170699)	170699..170989	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY54_RS00820 (171348)	171348..171626	+	279	WP_001296612.1	hypothetical protein	-
AOY54_RS00825 (172022)	172022..172240	+	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY54_RS00830 (172456)	172456..173385	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY54_RS00835 (173382)	173382..174017	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY54_RS00840 (174014)	174014..174916	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T104058 WP_000897302.1 NZ_CP028771:170387-170698 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T104058 NZ_CP028771:170387-170698 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT104058 WP_000356397.1 NZ_CP028771:170699-170989 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT104058 NZ_CP028771:170699-170989 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416