Detailed information of TA system TA104057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4789023..4789625
• Replicon: chromosome
• Accession: NZ_CP028770
• Organism: Escherichia coli strain A4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AOY55_RS22940
• Protein ID: WP_000897302.1
• Coordinates: 4789314..4789625 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY55_RS22935
• Protein ID: WP_000356397.1
• Coordinates: 4789023..4789313 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY55_RS22910 (4785096)	4785096..4785998	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY55_RS22915 (4785995)	4785995..4786630	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY55_RS22920 (4786627)	4786627..4787556	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY55_RS22925 (4787772)	4787772..4787990	-	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY55_RS22930 (4788386)	4788386..4788664	-	279	WP_001296612.1	hypothetical protein	-
AOY55_RS22935 (4789023)	4789023..4789313	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY55_RS22940 (4789314)	4789314..4789625	-	312	WP_000897302.1	hypothetical protein	Toxin
AOY55_RS22945 (4789854)	4789854..4790762	+	909	WP_001331553.1	alpha/beta hydrolase	-
AOY55_RS22950 (4790826)	4790826..4791767	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY55_RS22955 (4791812)	4791812..4792249	-	438	WP_000560990.1	D-aminoacyl-tRNA deacylase	-
AOY55_RS22960 (4792246)	4792246..4793118	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY55_RS22965 (4793112)	4793112..4793711	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T104057 WP_000897302.1 NZ_CP028770:c4789625-4789314 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T104057 NZ_CP028770:c4789625-4789314 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT104057 WP_000356397.1 NZ_CP028770:c4789313-4789023 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT104057 NZ_CP028770:c4789313-4789023 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416