Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3889606..3890300 Replicon chromosome
Accession NZ_CP028770
Organism Escherichia coli strain A4

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag AOY55_RS18775 Protein ID WP_001263501.1
Coordinates 3889606..3890004 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag AOY55_RS18780 Protein ID WP_000554755.1
Coordinates 3890007..3890300 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY55_RS18750 (3884971) 3884971..3885429 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
AOY55_RS18755 (3885690) 3885690..3887147 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
AOY55_RS18760 (3887204) 3887204..3887818 - 615 WP_000602123.1 peptide chain release factor H -
AOY55_RS18765 (3887815) 3887815..3888954 - 1140 WP_000521578.1 RNA ligase RtcB family protein -
AOY55_RS18770 (3889144) 3889144..3889596 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
AOY55_RS18775 (3889606) 3889606..3890004 - 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AOY55_RS18780 (3890007) 3890007..3890300 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AOY55_RS18785 (3890352) 3890352..3891407 - 1056 WP_001226170.1 DNA polymerase IV -
AOY55_RS18790 (3891478) 3891478..3892401 - 924 WP_001519504.1 putative lateral flagellar export/assembly protein LafU -
AOY55_RS18795 (3892404) 3892404..3893267 - 864 WP_001169519.1 flagellar motor stator protein MotA -
AOY55_RS18800 (3893280) 3893280..3893999 - 720 WP_000938737.1 FliA/WhiG family RNA polymerase sigma factor -
AOY55_RS18805 (3894019) 3894019..3894486 - 468 WP_000725264.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T104051 WP_001263501.1 NZ_CP028770:c3890004-3889606 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T104051 NZ_CP028770:c3890004-3889606 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT104051 WP_000554755.1 NZ_CP028770:c3890300-3890007 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT104051 NZ_CP028770:c3890300-3890007 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References