Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2651132..2651685 Replicon chromosome
Accession NZ_CP028770
Organism Escherichia coli strain A4

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag AOY55_RS12705 Protein ID WP_000605680.1
Coordinates 2651132..2651401 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY55_RS12710 Protein ID WP_000781370.1
Coordinates 2651401..2651685 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY55_RS12680 (2646718) 2646718..2647149 - 432 WP_000152305.1 peroxiredoxin OsmC -
AOY55_RS12685 (2647494) 2647494..2647709 + 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY55_RS12690 (2647811) 2647811..2647948 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY55_RS12695 (2648105) 2648105..2649802 + 1698 WP_000433464.1 malate dehydrogenase -
AOY55_RS12700 (2649936) 2649936..2650946 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AOY55_RS12705 (2651132) 2651132..2651401 + 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY55_RS12710 (2651401) 2651401..2651685 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY55_RS12715 (2651914) 2651914..2652567 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY55_RS12720 (2652560) 2652560..2653444 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AOY55_RS12725 (2653457) 2653457..2656504 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T104048 WP_000605680.1 NZ_CP028770:2651132-2651401 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T104048 NZ_CP028770:2651132-2651401 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT104048 WP_000781370.1 NZ_CP028770:2651401-2651685 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT104048 NZ_CP028770:2651401-2651685 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References