Detailed information of TA system TA104033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4611917..4612519
• Replicon: chromosome
• Accession: NZ_CP028769
• Organism: Escherichia coli strain A5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY56_RS22050
• Protein ID: WP_000897305.1
• Coordinates: 4612208..4612519 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY56_RS22045
• Protein ID: WP_000356397.1
• Coordinates: 4611917..4612207 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY56_RS22020 (4607463)	4607463..4608098	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY56_RS22025 (4608095)	4608095..4609024	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
AOY56_RS22030 (4609424)	4609424..4610404	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
AOY56_RS22035 (4610633)	4610633..4610875	-	243	WP_001086388.1	protein YiiF	-
AOY56_RS22040 (4611094)	4611094..4611312	-	219	WP_001295676.1	CopG family transcriptional regulator	-
AOY56_RS22045 (4611917)	4611917..4612207	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY56_RS22050 (4612208)	4612208..4612519	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY56_RS22055 (4612748)	4612748..4613655	+	908	Protein_4326	alpha/beta hydrolase	-
AOY56_RS22060 (4613719)	4613719..4614660	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY56_RS22065 (4614705)	4614705..4615142	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY56_RS22070 (4615139)	4615139..4616011	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY56_RS22075 (4616005)	4616005..4616604	-	600	WP_001295269.1	glucose-1-phosphatase	-
AOY56_RS22080 (4616703)	4616703..4617488	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4609424..4610404	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T104033 WP_000897305.1 NZ_CP028769:c4612519-4612208 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T104033 NZ_CP028769:c4612519-4612208 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT104033 WP_000356397.1 NZ_CP028769:c4612207-4611917 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT104033 NZ_CP028769:c4612207-4611917 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7