Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3765462..3766003 Replicon chromosome
Accession NZ_CP028769
Organism Escherichia coli strain A5

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY56_RS18195 Protein ID WP_000615976.1
Coordinates 3765725..3766003 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY56_RS18190 Protein ID WP_000729704.1
Coordinates 3765462..3765722 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY56_RS18165 (3761245) 3761245..3762030 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
AOY56_RS18170 (3762002) 3762002..3763714 + 1713 Protein_3572 flagellar biosynthesis protein FlhA -
AOY56_RS18175 (3763819) 3763819..3764097 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY56_RS18180 (3764090) 3764090..3764446 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY56_RS18185 (3764503) 3764503..3765276 - 774 WP_000543899.1 C40 family peptidase -
AOY56_RS18190 (3765462) 3765462..3765722 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY56_RS18195 (3765725) 3765725..3766003 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY56_RS18200 (3766159) 3766159..3766899 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY56_RS18205 (3766870) 3766870..3767637 - 768 WP_000333380.1 class II glutamine amidotransferase -
AOY56_RS18210 (3767843) 3767843..3768421 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T104028 WP_000615976.1 NZ_CP028769:3765725-3766003 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T104028 NZ_CP028769:3765725-3766003 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT104028 WP_000729704.1 NZ_CP028769:3765462-3765722 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT104028 NZ_CP028769:3765462-3765722 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References