Detailed information of TA system TA104013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1856837..1857339
• Replicon: chromosome
• Accession: NZ_CP028769
• Organism: Escherichia coli strain A5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: AOY56_RS08865
• Protein ID: WP_000767829.1
• Coordinates: 1857085..1857339 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: AOY56_RS08860
• Protein ID: WP_001259255.1
• Coordinates: 1856837..1857088 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY56_RS08835 (1852015)	1852015..1853082	-	1068	WP_023356487.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
AOY56_RS08840 (1853082)	1853082..1854152	-	1071	WP_000108962.1	histidinol-phosphate transaminase	-
AOY56_RS08845 (1854149)	1854149..1855453	-	1305	WP_001369878.1	histidinol dehydrogenase	-
AOY56_RS08850 (1855459)	1855459..1856358	-	900	WP_000131756.1	ATP phosphoribosyltransferase	-
AOY56_RS08855 (1856504)	1856504..1856554	-	51	WP_001364200.1	his operon leader peptide	-
AOY56_RS08860 (1856837)	1856837..1857088	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
AOY56_RS08865 (1857085)	1857085..1857339	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
AOY56_RS08870 (1857422)	1857422..1858246	+	825	WP_000754749.1	SDR family oxidoreductase	-
AOY56_RS08875 (1858292)	1858292..1859221	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
AOY56_RS08880 (1859436)	1859436..1859498	+	63	WP_010723108.1	membrane protein YoeI	-
AOY56_RS08885 (1859488)	1859488..1860846	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
AOY56_RS08890 (1861025)	1861025..1862083	+	1059	WP_000492305.1	thiosulfate utilization transporter TsuA/YeeE	-
AOY56_RS08895 (1862097)	1862097..1862324	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T104013 WP_000767829.1 NZ_CP028769:1857085-1857339 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T104013 NZ_CP028769:1857085-1857339 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT104013 WP_001259255.1 NZ_CP028769:1856837-1857088 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT104013 NZ_CP028769:1856837-1857088 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0