Detailed information of TA system TA104003

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4187750..4188162
• Replicon: chromosome
• Accession: NZ_CP028768
• Organism: Escherichia coli strain A6

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: AOY57_RS20710
• Protein ID: WP_000132614.1
• Coordinates: 4187821..4188162 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4187750..4187826 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY57_RS20700 (4184362)	4184362..4185831	+	1470	WP_001387312.1	type I restriction-modification system subunit M	-
AOY57_RS20705 (4185831)	4185831..4187600	+	1770	WP_201478626.1	restriction endonuclease subunit S	-
- (4187750)	4187750..4187826	-	77	NuclAT_12	-	Antitoxin
- (4187750)	4187750..4187826	-	77	NuclAT_12	-	Antitoxin
- (4187750)	4187750..4187826	-	77	NuclAT_12	-	Antitoxin
- (4187750)	4187750..4187826	-	77	NuclAT_12	-	Antitoxin
- (4187750)	4187750..4187826	-	77	NuclAT_13	-	Antitoxin
- (4187750)	4187750..4187826	-	77	NuclAT_13	-	Antitoxin
- (4187750)	4187750..4187826	-	77	NuclAT_13	-	Antitoxin
- (4187750)	4187750..4187826	-	77	NuclAT_13	-	Antitoxin
AOY57_RS20710 (4187821)	4187821..4188162	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
AOY57_RS20715 (4188209)	4188209..4189372	-	1164	WP_224153787.1	DUF1524 domain-containing protein	-
AOY57_RS20720 (4189426)	4189426..4190302	-	877	Protein_4070	DUF262 domain-containing protein	-
AOY57_RS20725 (4190708)	4190708..4191628	-	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
AOY57_RS20730 (4191813)	4191813..4193093	+	1281	WP_001338077.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4172138..4188162	16024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T104003 WP_000132614.1 NZ_CP028768:4187821-4188162 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T104003 NZ_CP028768:4187821-4188162 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT104003 NZ_CP028768:c4187826-4187750 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin