Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2599443..2599996 Replicon chromosome
Accession NZ_CP028768
Organism Escherichia coli strain A6

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag AOY57_RS12940 Protein ID WP_201478810.1
Coordinates 2599443..2599712 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY57_RS12945 Protein ID WP_000781370.1
Coordinates 2599712..2599996 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY57_RS12915 (2595029) 2595029..2595460 - 432 WP_000152307.1 peroxiredoxin OsmC -
AOY57_RS12920 (2595805) 2595805..2596020 + 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY57_RS12925 (2596122) 2596122..2596259 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY57_RS12930 (2596416) 2596416..2598113 + 1698 WP_000433460.1 malate dehydrogenase -
AOY57_RS12935 (2598247) 2598247..2599257 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AOY57_RS12940 (2599443) 2599443..2599712 + 270 WP_201478810.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY57_RS12945 (2599712) 2599712..2599996 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY57_RS12950 (2600225) 2600225..2600878 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY57_RS12955 (2600871) 2600871..2601755 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AOY57_RS12960 (2601768) 2601768..2604815 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10211.75 Da        Isoelectric Point: 6.8582

>T104000 WP_201478810.1 NZ_CP028768:2599443-2599712 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPSYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T104000 NZ_CP028768:2599443-2599712 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCAGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT104000 WP_000781370.1 NZ_CP028768:2599712-2599996 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT104000 NZ_CP028768:2599712-2599996 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References