Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1922747..1923249 Replicon chromosome
Accession NZ_CP028768
Organism Escherichia coli strain A6

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag AOY57_RS09305 Protein ID WP_000767828.1
Coordinates 1922995..1923249 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AOY57_RS09300 Protein ID WP_001259255.1
Coordinates 1922747..1922998 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY57_RS09275 (1917925) 1917925..1918992 - 1068 WP_000080129.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AOY57_RS09280 (1918992) 1918992..1920062 - 1071 WP_000108949.1 histidinol-phosphate transaminase -
AOY57_RS09285 (1920059) 1920059..1921363 - 1305 WP_024223692.1 histidinol dehydrogenase -
AOY57_RS09290 (1921369) 1921369..1922268 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AOY57_RS09295 (1922414) 1922414..1922464 - 51 WP_001364200.1 his operon leader peptide -
AOY57_RS09300 (1922747) 1922747..1922998 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AOY57_RS09305 (1922995) 1922995..1923249 + 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AOY57_RS09310 (1923332) 1923332..1924156 + 825 WP_000754740.1 SDR family oxidoreductase -
AOY57_RS09315 (1924202) 1924202..1925131 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
AOY57_RS09320 (1925346) 1925346..1925408 + 63 WP_010723108.1 membrane protein YoeI -
AOY57_RS09325 (1925398) 1925398..1926756 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AOY57_RS09330 (1926935) 1926935..1927993 + 1059 WP_201478515.1 thiosulfate utilization transporter TsuA/YeeE -
AOY57_RS09335 (1928007) 1928007..1928234 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T103988 WP_000767828.1 NZ_CP028768:1922995-1923249 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T103988 NZ_CP028768:1922995-1923249 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT103988 WP_001259255.1 NZ_CP028768:1922747-1922998 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT103988 NZ_CP028768:1922747-1922998 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References