Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 992234..992775 Replicon chromosome
Accession NZ_CP028767
Organism Escherichia coli strain A7

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag AOY58_RS04560 Protein ID WP_000615979.1
Coordinates 992234..992512 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY58_RS04565 Protein ID WP_000729704.1
Coordinates 992515..992775 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY58_RS04545 (989816) 989816..990394 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY58_RS04550 (990600) 990600..991367 + 768 WP_000333380.1 class II glutamine amidotransferase -
AOY58_RS04555 (991338) 991338..992078 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY58_RS04560 (992234) 992234..992512 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY58_RS04565 (992515) 992515..992775 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY58_RS04570 (992961) 992961..993734 + 774 WP_000543899.1 C40 family peptidase -
AOY58_RS04575 (993791) 993791..994147 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY58_RS04580 (994140) 994140..994418 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY58_RS04585 (994523) 994523..996235 - 1713 Protein_877 flagellar biosynthesis protein FlhA -
AOY58_RS04590 (996207) 996207..996992 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T103959 WP_000615979.1 NZ_CP028767:c992512-992234 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103959 NZ_CP028767:c992512-992234 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103959 WP_000729704.1 NZ_CP028767:c992775-992515 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103959 NZ_CP028767:c992775-992515 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References