Detailed information of TA system TA103957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 158709..159311
• Replicon: chromosome
• Accession: NZ_CP028767
• Organism: Escherichia coli strain A7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY58_RS00765
• Protein ID: WP_000897305.1
• Coordinates: 158709..159020 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY58_RS00770
• Protein ID: WP_000356397.1
• Coordinates: 159021..159311 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY58_RS00735 (153739)	153739..154524	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY58_RS00740 (154623)	154623..155222	+	600	WP_001295269.1	glucose-1-phosphatase	-
AOY58_RS00745 (155216)	155216..156088	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY58_RS00750 (156085)	156085..156522	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY58_RS00755 (156567)	156567..157508	+	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
AOY58_RS00760 (157572)	157572..158480	-	909	WP_001162704.1	alpha/beta hydrolase	-
AOY58_RS00765 (158709)	158709..159020	+	312	WP_000897305.1	hypothetical protein	Toxin
AOY58_RS00770 (159021)	159021..159311	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY58_RS00775 (159916)	159916..160134	+	219	WP_001297075.1	CopG family transcriptional regulator	-
AOY58_RS00780 (160353)	160353..160595	+	243	WP_001086388.1	protein YiiF	-
AOY58_RS00785 (160925)	160925..161854	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY58_RS00790 (161851)	161851..162486	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY58_RS00795 (162483)	162483..163385	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103957 WP_000897305.1 NZ_CP028767:158709-159020 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103957 NZ_CP028767:158709-159020 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103957 WP_000356397.1 NZ_CP028767:159021-159311 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103957 NZ_CP028767:159021-159311 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7