Detailed information of TA system TA103952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3802175..3802703
• Replicon: chromosome
• Accession: NZ_CP028766
• Organism: Escherichia coli strain A8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AOY61_RS18545
• Protein ID: WP_005080041.1
• Coordinates: 3802175..3802405 (+)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY61_RS18550
• Protein ID: WP_096979572.1
• Coordinates: 3802395..3802703 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY61_RS18520 (3798513)	3798513..3799403	-	891	WP_096979593.1	ATP-binding protein	-
AOY61_RS18525 (3799421)	3799421..3800332	-	912	WP_096979582.1	helix-turn-helix domain-containing protein	-
AOY61_RS18530 (3800329)	3800329..3800553	-	225	WP_096979579.1	hypothetical protein	-
AOY61_RS18535 (3800550)	3800550..3801209	-	660	WP_096979577.1	ash family protein	-
AOY61_RS18540 (3801206)	3801206..3802210	-	1005	WP_240774767.1	Rha family phage regulatory protein	-
AOY61_RS18545 (3802175)	3802175..3802405	+	231	WP_005080041.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOY61_RS18550 (3802395)	3802395..3802703	+	309	WP_096979572.1	helix-turn-helix transcriptional regulator	Antitoxin
AOY61_RS18555 (3802722)	3802722..3802898	-	177	WP_096979570.1	DUF4222 domain-containing protein	-
AOY61_RS18560 (3802918)	3802918..3803625	-	708	WP_096979568.1	Rha family transcriptional regulator	-
AOY61_RS18565 (3803707)	3803707..3803940	-	234	WP_000944728.1	YdaS family helix-turn-helix protein	-
AOY61_RS18570 (3804097)	3804097..3804786	+	690	WP_096979566.1	LexA family transcriptional regulator	-
AOY61_RS18575 (3804932)	3804932..3805624	+	693	WP_096979564.1	helix-turn-helix domain-containing protein	-
AOY61_RS18580 (3805630)	3805630..3805830	+	201	WP_096979561.1	hypothetical protein	-
AOY61_RS18585 (3806029)	3806029..3806211	+	183	WP_096979558.1	hypothetical protein	-
AOY61_RS18590 (3806217)	3806217..3806852	+	636	WP_096979556.1	hypothetical protein	-
AOY61_RS18595 (3807053)	3807053..3807331	+	279	WP_096979552.1	DUF4752 family protein	-
AOY61_RS18600 (3807450)	3807450..3807542	+	93	Protein_3633	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3766477..3834975	68498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (5-70)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8682.15 Da
• Isoelectric Point: 10.5308

>T103952 WP_005080041.1 NZ_CP028766:3802175-3802405 [Escherichia coli]
MKVYGSNLGEPHTSAFGDGLFELRIKGSDGIARVFYCTLTGKRIIMLHSFIKKTQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 231 bp

>T103952 NZ_CP028766:3802175-3802405 [Escherichia coli]
ATGAAAGTATACGGCAGCAACCTCGGAGAACCTCATACCAGCGCCTTCGGTGACGGACTTTTCGAGCTTCGGATTAAAGG
CAGTGATGGCATCGCACGCGTCTTTTACTGCACTCTGACAGGAAAACGCATCATCATGCTGCACAGTTTCATCAAGAAGA
CTCAGAAAACCCCACCGGCTGAACGCAAAAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11170.84 Da
• Isoelectric Point: 8.5840

>AT103952 WP_096979572.1 NZ_CP028766:3802395-3802703 [Escherichia coli]
MTGKRNPPTMTHDEMAAKWMDDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGVSG
PSFSTLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT103952 NZ_CP028766:3802395-3802703 [Escherichia coli]
ATGACTGGTAAACGTAATCCGCCAACCATGACACACGATGAAATGGCAGCTAAATGGATGGATGACCCGGCTTTTAAAGC
AGAATACGACGCCATCGCCGACGAGTTCGCACTGCTTGATGAAATGCTGGCCGCACGCAAAGAAGCTGGTCTCACCCAGG
CTGAAGTTGCTGAACGCATGGGAACTAAAGCAACTGCCATCACCAGAATGGAAAGCAATCTTGCCTCTGGTGTCAGTGGG
CCATCATTTTCCACACTGAAAAAATTTGCACGCGCGACCGGGAAAAAACTCCAAATCCGCTTCGTGTAA