103950

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3099271..3099773 Replicon chromosome
Accession NZ_CP028766
Organism Escherichia coli strain A8

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag AOY61_RS15215 Protein ID WP_000767828.1
Coordinates 3099271..3099525 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AOY61_RS15220 Protein ID WP_001259255.1
Coordinates 3099522..3099773 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY61_RS15185 (3094286) 3094286..3094513 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
AOY61_RS15190 (3094527) 3094527..3095585 - 1059 WP_000492344.1 thiosulfate utilization transporter TsuA/YeeE -
AOY61_RS15195 (3095764) 3095764..3097122 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AOY61_RS15200 (3097112) 3097112..3097174 - 63 WP_010723108.1 membrane protein YoeI -
AOY61_RS15205 (3097389) 3097389..3098318 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
AOY61_RS15210 (3098364) 3098364..3099188 - 825 WP_000754758.1 SDR family oxidoreductase -
AOY61_RS15215 (3099271) 3099271..3099525 - 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AOY61_RS15220 (3099522) 3099522..3099773 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AOY61_RS15225 (3100056) 3100056..3100106 + 51 WP_001364200.1 his operon leader peptide -
AOY61_RS15230 (3100252) 3100252..3101151 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AOY61_RS15235 (3101157) 3101157..3102461 + 1305 WP_032241630.1 histidinol dehydrogenase -
AOY61_RS15240 (3102458) 3102458..3103528 + 1071 WP_032241632.1 histidinol-phosphate transaminase -
AOY61_RS15245 (3103528) 3103528..3104595 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T103950 WP_000767828.1 NZ_CP028766:c3099525-3099271 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T103950 NZ_CP028766:c3099525-3099271 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT103950 WP_001259255.1 NZ_CP028766:c3099773-3099522 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT103950 NZ_CP028766:c3099773-3099522 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References