Detailed information of TA system TA103932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 152129..152731
• Replicon: chromosome
• Accession: NZ_CP028766
• Organism: Escherichia coli strain A8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY61_RS00740
• Protein ID: WP_000897305.1
• Coordinates: 152129..152440 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY61_RS00745
• Protein ID: WP_000356397.1
• Coordinates: 152441..152731 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY61_RS00710 (147159)	147159..147944	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY61_RS00715 (148043)	148043..148642	+	600	WP_001295269.1	glucose-1-phosphatase	-
AOY61_RS00720 (148636)	148636..149508	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY61_RS00725 (149505)	149505..149942	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY61_RS00730 (149987)	149987..150928	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY61_RS00735 (150992)	150992..151900	-	909	WP_001162704.1	alpha/beta hydrolase	-
AOY61_RS00740 (152129)	152129..152440	+	312	WP_000897305.1	hypothetical protein	Toxin
AOY61_RS00745 (152441)	152441..152731	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY61_RS00750 (153336)	153336..153554	+	219	WP_001295676.1	CopG family transcriptional regulator	-
AOY61_RS00755 (153773)	153773..154015	+	243	WP_001086388.1	protein YiiF	-
AOY61_RS00760 (154345)	154345..155274	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY61_RS00765 (155271)	155271..155906	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY61_RS00770 (155903)	155903..156805	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103932 WP_000897305.1 NZ_CP028766:152129-152440 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103932 NZ_CP028766:152129-152440 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103932 WP_000356397.1 NZ_CP028766:152441-152731 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103932 NZ_CP028766:152441-152731 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7