Detailed information of TA system TA103915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1149328..1150055
• Replicon: chromosome
• Accession: NZ_CP028765
• Organism: Escherichia coli strain A9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: AOY62_RS05470
• Protein ID: WP_000547555.1
• Coordinates: 1149328..1149639 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY62_RS05475
• Protein ID: WP_201477833.1
• Coordinates: 1149636..1150055 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY62_RS05445 (1145263)	1145263..1146972	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
AOY62_RS05450 (1146982)	1146982..1147524	+	543	WP_000493799.1	formate hydrogenlyase subunit HycF	-
AOY62_RS05455 (1147524)	1147524..1148291	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
AOY62_RS05460 (1148288)	1148288..1148698	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
AOY62_RS05465 (1148691)	1148691..1149161	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
AOY62_RS05470 (1149328)	1149328..1149639	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
AOY62_RS05475 (1149636)	1149636..1150055	+	420	WP_201477833.1	helix-turn-helix domain-containing protein	Antitoxin
AOY62_RS05480 (1150134)	1150134..1151558	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
AOY62_RS05485 (1151567)	1151567..1153024	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AOY62_RS05490 (1153284)	1153284..1154294	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
AOY62_RS05495 (1154443)	1154443..1154970	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T103915 WP_000547555.1 NZ_CP028765:1149328-1149639 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T103915 NZ_CP028765:1149328-1149639 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15415.48 Da
• Isoelectric Point: 4.4596

>AT103915 WP_201477833.1 NZ_CP028765:1149636-1150055 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDLEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYESNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT103915 NZ_CP028765:1149636-1150055 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTT
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAGCAACAACGAAAAATTTGCAGAGTTCGACAAAGCCGTTGCTGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2