Detailed information of TA system TA103912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 667767..668566
• Replicon: chromosome
• Accession: NZ_CP028765
• Organism: Escherichia coli strain A9

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: AOY62_RS03185
• Protein ID: WP_096977891.1
• Coordinates: 667767..668231 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A368IY36
• Locus tag: AOY62_RS03190
• Protein ID: WP_001604880.1
• Coordinates: 668231..668566 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY62_RS03155 (662768)	662768..663202	-	435	WP_000948829.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
AOY62_RS03160 (663220)	663220..664098	-	879	WP_201477895.1	PTS N-acetylgalactosamine transporter subunit IID	-
AOY62_RS03165 (664088)	664088..664867	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
AOY62_RS03170 (664878)	664878..665351	-	474	WP_201477894.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AOY62_RS03175 (665374)	665374..666654	-	1281	WP_000681955.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AOY62_RS03180 (666903)	666903..667712	+	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
AOY62_RS03185 (667767)	667767..668231	-	465	WP_096977891.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AOY62_RS03190 (668231)	668231..668566	-	336	WP_001604880.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AOY62_RS03195 (668715)	668715..670286	-	1572	WP_001273756.1	galactarate dehydratase	-
AOY62_RS03200 (670661)	670661..671995	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
AOY62_RS03205 (672011)	672011..672781	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17855.30 Da
• Isoelectric Point: 9.8488

>T103912 WP_096977891.1 NZ_CP028765:c668231-667767 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGRPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T103912 NZ_CP028765:c668231-667767 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACGTCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12340.91 Da
• Isoelectric Point: 4.8616

>AT103912 WP_001604880.1 NZ_CP028765:c668566-668231 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGLDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT103912 NZ_CP028765:c668566-668231 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACCATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCCTGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A368IY36