Detailed information of TA system TA103910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4824074..4824676
• Replicon: chromosome
• Accession: NZ_CP028764
• Organism: Escherichia coli strain A10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY63_RS23155
• Protein ID: WP_000897305.1
• Coordinates: 4824365..4824676 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY63_RS23150
• Protein ID: WP_000356395.1
• Coordinates: 4824074..4824364 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY63_RS23120 (4819697)	4819697..4820599	+	903	WP_201477237.1	formate dehydrogenase O subunit beta	-
AOY63_RS23125 (4820596)	4820596..4821231	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY63_RS23130 (4821228)	4821228..4822157	+	930	WP_000027710.1	formate dehydrogenase accessory protein FdhE	-
AOY63_RS23135 (4822339)	4822339..4822581	-	243	WP_001306649.1	protein YiiF	-
AOY63_RS23140 (4822800)	4822800..4823018	-	219	WP_001295676.1	CopG family transcriptional regulator	-
AOY63_RS23145 (4823437)	4823437..4823715	-	279	WP_001306650.1	hypothetical protein	-
AOY63_RS23150 (4824074)	4824074..4824364	-	291	WP_000356395.1	NadS family protein	Antitoxin
AOY63_RS23155 (4824365)	4824365..4824676	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY63_RS23160 (4824905)	4824905..4825813	+	909	WP_001306651.1	alpha/beta hydrolase	-
AOY63_RS23165 (4825982)	4825982..4826896	-	915	WP_240751381.1	transposase	-
AOY63_RS23170 (4826909)	4826909..4827796	-	888	Protein_4541	hypothetical protein	-
AOY63_RS23175 (4828212)	4828212..4829153	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY63_RS23180 (4829198)	4829198..4829635	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103910 WP_000897305.1 NZ_CP028764:c4824676-4824365 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103910 NZ_CP028764:c4824676-4824365 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT103910 WP_000356395.1 NZ_CP028764:c4824364-4824074 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103910 NZ_CP028764:c4824364-4824074 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7