Detailed information of TA system TA103904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3844666..3845316
• Replicon: chromosome
• Accession: NZ_CP028764
• Organism: Escherichia coli strain A10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AOY63_RS18445
• Protein ID: WP_000263532.1
• Coordinates: 3844975..3845316 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: D3GSB3
• Locus tag: AOY63_RS18440
• Protein ID: WP_000212551.1
• Coordinates: 3844666..3844965 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY63_RS18410 (3840114)	3840114..3840425	-	312	WP_000415006.1	hypothetical protein	-
AOY63_RS18415 (3840430)	3840430..3840822	-	393	WP_000285319.1	flagellar export chaperone FliS	-
AOY63_RS18420 (3840845)	3840845..3842161	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
AOY63_RS18425 (3842154)	3842154..3842366	-	213	WP_000774464.1	hypothetical protein	-
AOY63_RS18430 (3842366)	3842366..3843280	-	915	WP_000949083.1	lateral flagellin LafA	-
AOY63_RS18435 (3843766)	3843766..3844608	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
AOY63_RS18440 (3844666)	3844666..3844965	-	300	WP_000212551.1	XRE family transcriptional regulator	Antitoxin
AOY63_RS18445 (3844975)	3844975..3845316	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOY63_RS18450 (3845392)	3845392..3846369	-	978	WP_001195938.1	hypothetical protein	-
AOY63_RS18455 (3846386)	3846386..3847315	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
AOY63_RS18460 (3847330)	3847330..3848706	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
AOY63_RS18465 (3848882)	3848882..3849181	-	300	WP_000867281.1	rod-binding protein	-
AOY63_RS18470 (3849181)	3849181..3850281	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T103904 WP_000263532.1 NZ_CP028764:c3845316-3844975 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T103904 NZ_CP028764:c3845316-3844975 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10976.81 Da
• Isoelectric Point: 9.7665

>AT103904 WP_000212551.1 NZ_CP028764:c3844965-3844666 [Escherichia coli]
MATLKELMAKQSPESQARIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT103904 NZ_CP028764:c3844965-3844666 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGCGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: D3GSB3