Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2538547..2539109 Replicon chromosome
Accession NZ_CP028764
Organism Escherichia coli strain A10

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag AOY63_RS12030 Protein ID WP_000605676.1
Coordinates 2538547..2538825 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY63_RS12035 Protein ID WP_000781370.1
Coordinates 2538825..2539109 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY63_RS12005 (2534133) 2534133..2534564 - 432 WP_000152305.1 peroxiredoxin OsmC -
AOY63_RS12010 (2534909) 2534909..2535124 + 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY63_RS12015 (2535226) 2535226..2535363 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY63_RS12020 (2535520) 2535520..2537217 + 1698 WP_000433462.1 malate dehydrogenase -
AOY63_RS12025 (2537351) 2537351..2538361 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
AOY63_RS12030 (2538547) 2538547..2538825 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY63_RS12035 (2538825) 2538825..2539109 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY63_RS12040 (2539337) 2539337..2539990 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
AOY63_RS12045 (2539983) 2539983..2540867 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
AOY63_RS12050 (2540880) 2540880..2543927 - 3048 WP_077786070.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T103898 WP_000605676.1 NZ_CP028764:2538547-2538825 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T103898 NZ_CP028764:2538547-2538825 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103898 WP_000781370.1 NZ_CP028764:2538825-2539109 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103898 NZ_CP028764:2538825-2539109 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References