Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1959473..1959975 Replicon chromosome
Accession NZ_CP028764
Organism Escherichia coli strain A10

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag AOY63_RS09065 Protein ID WP_000767820.1
Coordinates 1959721..1959975 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AOY63_RS09060 Protein ID WP_001259255.1
Coordinates 1959473..1959724 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY63_RS09035 (1954650) 1954650..1955717 - 1068 WP_000080071.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AOY63_RS09040 (1955717) 1955717..1956787 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
AOY63_RS09045 (1956784) 1956784..1958088 - 1305 WP_001306412.1 histidinol dehydrogenase -
AOY63_RS09050 (1958094) 1958094..1958993 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
AOY63_RS09055 (1959139) 1959139..1959189 - 51 WP_001364200.1 his operon leader peptide -
AOY63_RS09060 (1959473) 1959473..1959724 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AOY63_RS09065 (1959721) 1959721..1959975 + 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AOY63_RS09070 (1960058) 1960058..1960882 + 825 WP_000754757.1 SDR family oxidoreductase -
AOY63_RS09075 (1960928) 1960928..1961858 + 931 Protein_1790 LysR substrate-binding domain-containing protein -
AOY63_RS09080 (1962074) 1962074..1962136 + 63 WP_010723108.1 membrane protein YoeI -
AOY63_RS09085 (1962126) 1962126..1963484 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T103889 WP_000767820.1 NZ_CP028764:1959721-1959975 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T103889 NZ_CP028764:1959721-1959975 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT103889 WP_001259255.1 NZ_CP028764:1959473-1959724 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT103889 NZ_CP028764:1959473-1959724 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References