Detailed information of TA system TA103884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4613189..4613791
• Replicon: chromosome
• Accession: NZ_CP028763
• Organism: Escherichia coli strain A11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY64_RS22150
• Protein ID: WP_000897305.1
• Coordinates: 4613480..4613791 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY64_RS22145
• Protein ID: WP_000356397.1
• Coordinates: 4613189..4613479 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY64_RS22120 (4608736)	4608736..4609371	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY64_RS22125 (4609368)	4609368..4610297	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY64_RS22130 (4610696)	4610696..4611676	+	981	WP_094029558.1	IS110-like element IS621 family transposase	-
AOY64_RS22135 (4611905)	4611905..4612147	-	243	WP_001086388.1	protein YiiF	-
AOY64_RS22140 (4612366)	4612366..4612584	-	219	WP_001295676.1	CopG family transcriptional regulator	-
AOY64_RS22145 (4613189)	4613189..4613479	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY64_RS22150 (4613480)	4613480..4613791	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY64_RS22155 (4614020)	4614020..4614928	+	909	WP_001162704.1	alpha/beta hydrolase	-
AOY64_RS22160 (4614992)	4614992..4615933	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
AOY64_RS22165 (4615978)	4615978..4616415	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY64_RS22170 (4616412)	4616412..4617284	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY64_RS22175 (4617278)	4617278..4617877	-	600	WP_001315111.1	glucose-1-phosphatase	-
AOY64_RS22180 (4617976)	4617976..4618761	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4610696..4611676	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103884 WP_000897305.1 NZ_CP028763:c4613791-4613480 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103884 NZ_CP028763:c4613791-4613480 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103884 WP_000356397.1 NZ_CP028763:c4613479-4613189 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103884 NZ_CP028763:c4613479-4613189 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7