Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3794072..3794613 Replicon chromosome
Accession NZ_CP028763
Organism Escherichia coli strain A11

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY64_RS18385 Protein ID WP_000615976.1
Coordinates 3794335..3794613 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY64_RS18380 Protein ID WP_000729704.1
Coordinates 3794072..3794332 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY64_RS18355 (3789855) 3789855..3790640 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
AOY64_RS18360 (3790612) 3790612..3792324 + 1713 Protein_3604 flagellar biosynthesis protein FlhA -
AOY64_RS18365 (3792429) 3792429..3792707 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY64_RS18370 (3792700) 3792700..3793056 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY64_RS18375 (3793113) 3793113..3793886 - 774 WP_000543899.1 C40 family peptidase -
AOY64_RS18380 (3794072) 3794072..3794332 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY64_RS18385 (3794335) 3794335..3794613 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY64_RS18390 (3794769) 3794769..3795509 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY64_RS18395 (3795480) 3795480..3796247 - 768 WP_000333380.1 class II glutamine amidotransferase -
AOY64_RS18400 (3796453) 3796453..3797031 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T103881 WP_000615976.1 NZ_CP028763:3794335-3794613 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103881 NZ_CP028763:3794335-3794613 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103881 WP_000729704.1 NZ_CP028763:3794072-3794332 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103881 NZ_CP028763:3794072-3794332 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References