Detailed information of TA system TA103862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4754032..4754634
• Replicon: chromosome
• Accession: NZ_CP028762
• Organism: Escherichia coli strain A12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY65_RS22875
• Protein ID: WP_000897305.1
• Coordinates: 4754323..4754634 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY65_RS22870
• Protein ID: WP_000356397.1
• Coordinates: 4754032..4754322 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY65_RS22845 (4749578)	4749578..4750213	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY65_RS22850 (4750210)	4750210..4751139	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
AOY65_RS22855 (4751539)	4751539..4752519	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
AOY65_RS22860 (4752748)	4752748..4752990	-	243	WP_001087409.1	protein YiiF	-
AOY65_RS22865 (4753209)	4753209..4753427	-	219	WP_001295676.1	CopG family transcriptional regulator	-
AOY65_RS22870 (4754032)	4754032..4754322	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY65_RS22875 (4754323)	4754323..4754634	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY65_RS22880 (4754863)	4754863..4755771	+	909	WP_001162704.1	alpha/beta hydrolase	-
AOY65_RS22885 (4755835)	4755835..4756776	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY65_RS22890 (4756821)	4756821..4757258	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY65_RS22895 (4757255)	4757255..4758127	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY65_RS22900 (4758121)	4758121..4758720	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4751539..4752519	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103862 WP_000897305.1 NZ_CP028762:c4754634-4754323 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103862 NZ_CP028762:c4754634-4754323 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103862 WP_000356397.1 NZ_CP028762:c4754322-4754032 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103862 NZ_CP028762:c4754322-4754032 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7