Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1295829..1296508 Replicon chromosome
Accession NZ_CP028761
Organism Escherichia coli strain A13

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag AOY66_RS06080 Protein ID WP_000057524.1
Coordinates 1295829..1296131 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag AOY66_RS06085 Protein ID WP_000806442.1
Coordinates 1296167..1296508 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY66_RS06055 (1291206) 1291206..1292857 + 1652 Protein_1167 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
AOY66_RS06060 (1292895) 1292895..1293398 - 504 WP_000667000.1 hypothetical protein -
AOY66_RS06065 (1293395) 1293395..1294195 - 801 WP_000439798.1 hypothetical protein -
AOY66_RS06070 (1294219) 1294219..1294698 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
AOY66_RS06075 (1294902) 1294902..1295696 - 795 WP_000365177.1 TraB/GumN family protein -
AOY66_RS06080 (1295829) 1295829..1296131 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY66_RS06085 (1296167) 1296167..1296508 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
AOY66_RS06090 (1296566) 1296566..1299070 - 2505 WP_000084000.1 copper-exporting P-type ATPase CopA -
AOY66_RS06095 (1299333) 1299333..1300265 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T103815 WP_000057524.1 NZ_CP028761:1295829-1296131 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T103815 NZ_CP028761:1295829-1296131 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT103815 WP_000806442.1 NZ_CP028761:1296167-1296508 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT103815 NZ_CP028761:1296167-1296508 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References