Detailed information of TA system TA103813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1068015..1068709
• Replicon: chromosome
• Accession: NZ_CP028761
• Organism: Escherichia coli strain A13

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AOY66_RS05030
• Protein ID: WP_001263500.1
• Coordinates: 1068311..1068709 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AOY66_RS05025
• Protein ID: WP_000554758.1
• Coordinates: 1068015..1068308 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY66_RS05005 (1063751)	1063751..1064248	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
AOY66_RS05010 (1064368)	1064368..1066080	-	1713	Protein_959	flagellar biosynthesis protein FlhA	-
AOY66_RS05015 (1066052)	1066052..1066837	+	786	WP_000207543.1	putative lateral flagellar export/assembly protein LafU	-
AOY66_RS05020 (1066908)	1066908..1067963	+	1056	WP_001226162.1	DNA polymerase IV	-
AOY66_RS05025 (1068015)	1068015..1068308	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AOY66_RS05030 (1068311)	1068311..1068709	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AOY66_RS05035 (1068719)	1068719..1069171	+	453	WP_001059897.1	GNAT family N-acetyltransferase	-
AOY66_RS05040 (1069361)	1069361..1070500	+	1140	WP_000521564.1	RNA ligase RtcB family protein	-
AOY66_RS05045 (1070497)	1070497..1071111	+	615	WP_000602123.1	peptide chain release factor H	-
AOY66_RS05050 (1071168)	1071168..1072625	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
AOY66_RS05055 (1072886)	1072886..1073344	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1048690..1069171	20481

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T103813 WP_001263500.1 NZ_CP028761:1068311-1068709 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T103813 NZ_CP028761:1068311-1068709 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT103813 WP_000554758.1 NZ_CP028761:1068015-1068308 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT103813 NZ_CP028761:1068015-1068308 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3