Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1017229..1017770 Replicon chromosome
Accession NZ_CP028760
Organism Escherichia coli strain A14

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag AOY67_RS04865 Protein ID WP_000615983.1
Coordinates 1017229..1017507 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag AOY67_RS04870 Protein ID WP_000729703.1
Coordinates 1017510..1017770 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY67_RS04850 (1014811) 1014811..1015389 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY67_RS04855 (1015595) 1015595..1016362 + 768 WP_000333380.1 class II glutamine amidotransferase -
AOY67_RS04860 (1016333) 1016333..1017073 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY67_RS04865 (1017229) 1017229..1017507 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY67_RS04870 (1017510) 1017510..1017770 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY67_RS04875 (1017980) 1017980..1018729 + 750 WP_000056849.1 C40 family peptidase -
AOY67_RS04880 (1018905) 1018905..1019402 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
AOY67_RS04885 (1019626) 1019626..1021719 - 2094 WP_201488016.1 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - hcp1/tssD1 / tssA / rhs/PAAR / gmhA/lpcA 997891..1019402 21511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T103782 WP_000615983.1 NZ_CP028760:c1017507-1017229 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103782 NZ_CP028760:c1017507-1017229 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT103782 WP_000729703.1 NZ_CP028760:c1017770-1017510 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103782 NZ_CP028760:c1017770-1017510 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References