Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2978978..2979480 Replicon chromosome
Accession NZ_CP028757
Organism Escherichia coli strain A17

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag AOY70_RS14510 Protein ID WP_000767828.1
Coordinates 2978978..2979232 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AOY70_RS14515 Protein ID WP_001259255.1
Coordinates 2979229..2979480 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY70_RS14480 (2973993) 2973993..2974220 - 228 WP_000985280.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
AOY70_RS14485 (2974234) 2974234..2975292 - 1059 WP_000492318.1 thiosulfate utilization transporter TsuA/YeeE -
AOY70_RS14490 (2975471) 2975471..2976829 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AOY70_RS14495 (2976819) 2976819..2976881 - 63 WP_010723108.1 membrane protein YoeI -
AOY70_RS14500 (2977096) 2977096..2978025 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
AOY70_RS14505 (2978071) 2978071..2978895 - 825 WP_000754758.1 SDR family oxidoreductase -
AOY70_RS14510 (2978978) 2978978..2979232 - 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AOY70_RS14515 (2979229) 2979229..2979480 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AOY70_RS14520 (2979763) 2979763..2979813 + 51 WP_001364200.1 his operon leader peptide -
AOY70_RS14525 (2979959) 2979959..2980858 + 900 WP_000131783.1 ATP phosphoribosyltransferase -
AOY70_RS14530 (2980864) 2980864..2982168 + 1305 WP_063117142.1 histidinol dehydrogenase -
AOY70_RS14535 (2982165) 2982165..2983235 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
AOY70_RS14540 (2983235) 2983235..2984302 + 1068 WP_001567686.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T103726 WP_000767828.1 NZ_CP028757:c2979232-2978978 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T103726 NZ_CP028757:c2979232-2978978 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT103726 WP_001259255.1 NZ_CP028757:c2979480-2979229 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT103726 NZ_CP028757:c2979480-2979229 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References