Detailed information of TA system TA103712

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2273518..2273889
• Replicon: chromosome
• Accession: NZ_CP028757
• Organism: Escherichia coli strain A17

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: AOY70_RS10965
• Protein ID: WP_021500490.1
• Coordinates: 2273695..2273889 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2273518..2273696 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY70_RS10935 (2269270)	2269270..2269443	+	174	WP_001296046.1	protein YnaL	-
AOY70_RS10940 (2269473)	2269473..2270846	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
AOY70_RS10945 (2270975)	2270975..2271910	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
AOY70_RS10950 (2271962)	2271962..2273197	-	1236	WP_023568506.1	site-specific integrase	-
AOY70_RS10955 (2273199)	2273199..2273414	-	216	WP_000079604.1	excisionase XisR	-
- (2273518)	2273518..2273696	+	179	NuclAT_0	-	Antitoxin
- (2273518)	2273518..2273696	+	179	NuclAT_0	-	Antitoxin
- (2273518)	2273518..2273696	+	179	NuclAT_0	-	Antitoxin
- (2273518)	2273518..2273696	+	179	NuclAT_0	-	Antitoxin
AOY70_RS10960 (2273514)	2273514..2273702	-	189	WP_001298826.1	DUF1187 family protein	-
AOY70_RS10965 (2273695)	2273695..2273889	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
AOY70_RS10970 (2273953)	2273953..2275005	-	1053	WP_001004422.1	RecT family recombinase	-
AOY70_RS10975 (2275017)	2275017..2276048	-	1032	WP_240763307.1	PD-(D/E)XK nuclease-like domain-containing protein	-
AOY70_RS10980 (2276211)	2276211..2278088	-	1878	Protein_2136	exonuclease VIII	-
AOY70_RS10985 (2278190)	2278190..2278464	-	275	Protein_2137	hypothetical protein	-
AOY70_RS10990 (2278539)	2278539..2278709	-	171	WP_001349883.1	YdaE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2271962..2317565	45603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T103712 WP_021500490.1 NZ_CP028757:c2273889-2273695 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T103712 NZ_CP028757:c2273889-2273695 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT103712 NZ_CP028757:2273518-2273696 [Escherichia coli]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin