Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1030204..1030745 Replicon chromosome
Accession NZ_CP028757
Organism Escherichia coli strain A17

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY70_RS04815 Protein ID WP_000615976.1
Coordinates 1030204..1030482 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY70_RS04820 Protein ID WP_000729704.1
Coordinates 1030485..1030745 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY70_RS04800 (1027786) 1027786..1028364 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY70_RS04805 (1028570) 1028570..1029337 + 768 WP_000333380.1 class II glutamine amidotransferase -
AOY70_RS04810 (1029308) 1029308..1030048 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY70_RS04815 (1030204) 1030204..1030482 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY70_RS04820 (1030485) 1030485..1030745 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY70_RS04825 (1030931) 1030931..1031704 + 774 WP_000543895.1 C40 family peptidase -
AOY70_RS04830 (1031761) 1031761..1032117 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY70_RS04835 (1032110) 1032110..1032388 - 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
AOY70_RS04840 (1032493) 1032493..1034205 - 1713 Protein_931 flagellar biosynthesis protein FlhA -
AOY70_RS04845 (1034177) 1034177..1034962 + 786 WP_000207557.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / rhs/PAAR / gmhA/lpcA 1014790..1032388 17598


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T103709 WP_000615976.1 NZ_CP028757:c1030482-1030204 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103709 NZ_CP028757:c1030482-1030204 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103709 WP_000729704.1 NZ_CP028757:c1030745-1030485 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103709 NZ_CP028757:c1030745-1030485 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References