Detailed information of TA system TA103706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4851583..4852185
• Replicon: chromosome
• Accession: NZ_CP028756
• Organism: Escherichia coli strain A19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY72_RS23430
• Protein ID: WP_000897305.1
• Coordinates: 4851874..4852185 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY72_RS23425
• Protein ID: WP_000356397.1
• Coordinates: 4851583..4851873 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY72_RS23400 (4847130)	4847130..4847765	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY72_RS23405 (4847762)	4847762..4848691	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY72_RS23410 (4849090)	4849090..4850070	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
AOY72_RS23415 (4850299)	4850299..4850541	-	243	WP_001086388.1	protein YiiF	-
AOY72_RS23420 (4850760)	4850760..4850978	-	219	WP_001295676.1	CopG family transcriptional regulator	-
AOY72_RS23425 (4851583)	4851583..4851873	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY72_RS23430 (4851874)	4851874..4852185	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY72_RS23435 (4852414)	4852414..4853322	+	909	WP_001162704.1	alpha/beta hydrolase	-
AOY72_RS23440 (4853386)	4853386..4854327	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
AOY72_RS23445 (4854372)	4854372..4854809	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY72_RS23450 (4854806)	4854806..4855678	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY72_RS23455 (4855672)	4855672..4856271	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4849090..4850070	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103706 WP_000897305.1 NZ_CP028756:c4852185-4851874 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103706 NZ_CP028756:c4852185-4851874 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103706 WP_000356397.1 NZ_CP028756:c4851873-4851583 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103706 NZ_CP028756:c4851873-4851583 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7