103705

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4576848..4577345 Replicon chromosome
Accession NZ_CP028756
Organism Escherichia coli strain A19

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY72_RS22215 Protein ID WP_001173343.1
Coordinates 4576848..4577021 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AOY72_RS22220 Protein ID WP_000398619.1
Coordinates 4577049..4577345 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY72_RS22205 (4573518) 4573518..4574975 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
AOY72_RS22210 (4575212) 4575212..4576729 + 1518 WP_001295074.1 lysine--tRNA ligase -
AOY72_RS22215 (4576848) 4576848..4577021 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY72_RS22220 (4577049) 4577049..4577345 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY72_RS22225 (4577572) 4577572..4577844 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY72_RS22230 (4577856) 4577856..4578086 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
AOY72_RS22235 (4578267) 4578267..4579898 + 1632 WP_001216487.1 sensor histidine kinase -
AOY72_RS22240 (4579895) 4579895..4580614 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4566677..4577345 10668


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T103705 WP_001173343.1 NZ_CP028756:c4577021-4576848 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T103705 NZ_CP028756:c4577021-4576848 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT103705 WP_000398619.1 NZ_CP028756:c4577345-4577049 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT103705 NZ_CP028756:c4577345-4577049 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References