Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3875132..3875673 Replicon chromosome
Accession NZ_CP028756
Organism Escherichia coli strain A19

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY72_RS18820 Protein ID WP_000615976.1
Coordinates 3875395..3875673 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY72_RS18815 Protein ID WP_000729704.1
Coordinates 3875132..3875392 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY72_RS18790 (3870915) 3870915..3871700 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
AOY72_RS18795 (3871672) 3871672..3873384 + 1713 Protein_3686 flagellar biosynthesis protein FlhA -
AOY72_RS18800 (3873489) 3873489..3873767 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY72_RS18805 (3873760) 3873760..3874116 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY72_RS18810 (3874173) 3874173..3874946 - 774 WP_000543897.1 C40 family peptidase -
AOY72_RS18815 (3875132) 3875132..3875392 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY72_RS18820 (3875395) 3875395..3875673 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY72_RS18825 (3875829) 3875829..3876569 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY72_RS18830 (3876540) 3876540..3877307 - 768 WP_000333380.1 class II glutamine amidotransferase -
AOY72_RS18835 (3877513) 3877513..3878091 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T103702 WP_000615976.1 NZ_CP028756:3875395-3875673 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103702 NZ_CP028756:3875395-3875673 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103702 WP_000729704.1 NZ_CP028756:3875132-3875392 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103702 NZ_CP028756:3875132-3875392 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References