Detailed information of TA system TA103683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4814612..4815214
• Replicon: chromosome
• Accession: NZ_CP028755
• Organism: Escherichia coli strain A20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U9FTF1
• Locus tag: AOY73_RS23340
• Protein ID: WP_000897301.1
• Coordinates: 4814903..4815214 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY73_RS23335
• Protein ID: WP_000356397.1
• Coordinates: 4814612..4814902 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY73_RS23310 (4810685)	4810685..4811587	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY73_RS23315 (4811584)	4811584..4812219	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY73_RS23320 (4812216)	4812216..4813145	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY73_RS23325 (4813361)	4813361..4813579	-	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY73_RS23330 (4813975)	4813975..4814253	-	279	WP_001296612.1	hypothetical protein	-
AOY73_RS23335 (4814612)	4814612..4814902	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY73_RS23340 (4814903)	4814903..4815214	-	312	WP_000897301.1	hypothetical protein	Toxin
AOY73_RS23345 (4815451)	4815451..4816671	-	1221	WP_096980620.1	ISL3 family transposase	-
AOY73_RS23350 (4816767)	4816767..4817675	+	909	WP_001331553.1	alpha/beta hydrolase	-
AOY73_RS23355 (4817739)	4817739..4818680	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY73_RS23360 (4818725)	4818725..4819162	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY73_RS23365 (4819159)	4819159..4820031	-	873	WP_000920762.1	virulence factor BrkB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4815451..4816671	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12150.14 Da
• Isoelectric Point: 8.8941

>T103683 WP_000897301.1 NZ_CP028755:c4815214-4814903 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKCAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103683 NZ_CP028755:c4815214-4814903 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAATGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103683 WP_000356397.1 NZ_CP028755:c4814902-4814612 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103683 NZ_CP028755:c4814902-4814612 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U9FTF1