Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3889436..3889977 Replicon chromosome
Accession NZ_CP028755
Organism Escherichia coli strain A20

Toxin (Protein)


Gene name yafQ Uniprot ID A0A8H9JLL5
Locus tag AOY73_RS19015 Protein ID WP_042023972.1
Coordinates 3889699..3889977 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY73_RS19010 Protein ID WP_000729704.1
Coordinates 3889436..3889696 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY73_RS18985 (3884472) 3884472..3885611 + 1140 WP_000785667.1 flagellar type III secretion system protein FlhB -
AOY73_RS18990 (3885595) 3885595..3887688 + 2094 WP_087890322.1 flagellar biosynthesis protein FlhA -
AOY73_RS18995 (3887793) 3887793..3888071 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY73_RS19000 (3888064) 3888064..3888420 + 357 WP_001030482.1 type II toxin-antitoxin system HicB family antitoxin -
AOY73_RS19005 (3888477) 3888477..3889250 - 774 WP_122998384.1 C40 family peptidase -
AOY73_RS19010 (3889436) 3889436..3889696 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY73_RS19015 (3889699) 3889699..3889977 + 279 WP_042023972.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY73_RS19020 (3890133) 3890133..3890873 + 741 WP_096980312.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY73_RS19025 (3890844) 3890844..3891611 - 768 WP_000333380.1 class II glutamine amidotransferase -
AOY73_RS19030 (3891816) 3891816..3892394 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3888064..3921934 33870


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10831.61 Da        Isoelectric Point: 9.8881

>T103679 WP_042023972.1 NZ_CP028755:3889699-3889977 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINDTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103679 NZ_CP028755:3889699-3889977 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATGATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAGCCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103679 WP_000729704.1 NZ_CP028755:3889436-3889696 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103679 NZ_CP028755:3889436-3889696 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References